Creative Biogene’s Easy-Change® XL Site-Directed Mutagenesis Kit allows site specific mutation in virtually any double-stranded plasmid, thus eliminating the need for subcloning into M13-based bacteriophage vectors and for ssDNA rescue. If you want to know more about it, please kindly contact Creative Biogene at 1-631-626-9181 or visit http://www.creative-biogene.com/Easy-Change%C2%AE-XL-Site-Directed-Mutagenesis-Kit-SMK-Y001-1232919-77.html.